February 24th 2022

White paper published: High throughput cryo-electron microscopy epitope mapping of SARS-CoV-2 spike protein antibodies

High throughput cryo-electron microscopy epitope mapping of SARS-CoV-2 spike protein antibodies

The CARE partners Takeda and Utrecht University, in collaboration with Thermo Fisher Scientific, published a white paper demonstrating how cryo-electron microscopy can be used as tool for high-throughput epitope mapping of antibodies that block SARS-CoV-2 virus entry. One of the main concerns of the ongoing COVID-19 pandemic is SARS-CoV-2 variants that may be able to evade immunity. Epitope mapping of large antibody panels allows researchers to identify vulnerable sites on the spike protein, predict and interpret the effect of new mutations, and speed up decision-making in selecting antibody combinations that target non-overlapping epitopes. The results show how an optimized sample preparation workflow and state-of-the-art data technology can be used to expedite antibody drug discovery, which is of great importance for COVID-19 and future pandemic threats.

To learn more, click here: High-throughput cryo-EM epitope mapping of SARS- CoV-2 spike protein antibodies using EPU Multigrid